3E3J
Crystal Structure of an Intermediate Complex of T7 RNAP and 8nt of RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77.4 |
| Detector technology | CCD |
| Collection date | 2007-12-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.216, 180.602, 198.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.810 - 6.700 |
| R-factor | 0.39486 |
| Rwork | 0.392 |
| R-free | 0.45419 |
| Starting model (for MR) | 3e2e |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 6.940 |
| High resolution limit [Å] | 6.700 | 14.330 | 6.700 |
| Rmerge | 0.152 | 0.123 | 0.601 |
| Number of reflections | 6015 | ||
| Completeness [%] | 87.8 | 97.8 | 39.7 |
| Redundancy | 3.5 | 4.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 285 | PEG 6000, MgCl2, Tris ph8.5 at 285K, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 2 | PEG 6000 | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 2 | MgCl2 | ||
| 5 | 1 | 1 | Tris | ||
| 6 | 1 | 2 | Tris |
