3E0V
Crystal structure of pyruvate kinase from Leishmania mexicana in complex with sulphate ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 261.955, 261.955, 185.541 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.060 - 3.300 |
| R-factor | 0.22987 |
| Rwork | 0.227 |
| R-free | 0.28958 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pkl chain G |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.244 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.060 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.075 | 0.496 |
| Number of reflections | 86237 | |
| <I/σ(I)> | 8.9 | 1.6 |
| Completeness [%] | 89.4 | 89.4 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 277 | 20% Saturated ammonium sulphate, 100mM Na citrate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
