3E0N

The X-ray structure of Human Prostasin in complex with DFFR-chloromethyl ketone inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	37.677, 75.880, 79.179
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.1593
Rwork	0.157
R-free	0.20150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a0l
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.096	0.491
Number of reflections	28770
<I/σ(I)>	7.6	1.8
Completeness [%]	98.1	89.9
Redundancy	4.5	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1