3DXT
Crystal structure of the catalytic core domain of JMJD2D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 130 |
Collection date | 2007-02-28 |
Wavelength(s) | 1.00 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.630, 71.630, 151.295 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.240 - 1.800 |
R-factor | 0.17554 |
Rwork | 0.174 |
R-free | 0.20310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | jmjd2a |
RMSD bond length | 0.016 |
RMSD bond angle | 1.493 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 34305 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | hepes, 2.0M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |