3D12
Crystal Structures of Nipah Virus G Attachment Glycoprotein in Complex with its Receptor Ephrin-B3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Collection date | 2007-10-30 |
| Wavelength(s) | 0.9192 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 189.492, 189.492, 277.052 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.337 - 3.005 |
| Rwork | 0.221 |
| R-free | 0.26500 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.927 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.086 | 0.029 | 0.469 |
| Number of reflections | 58204 | ||
| <I/σ(I)> | 8.6 | ||
| Completeness [%] | 98.9 | 98.6 | 98.1 |
| Redundancy | 3.6 | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.5M Li2SO4 and 100mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
