3CZU
Crystal structure of the human ephrin A2- ephrin A1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-10 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 99.431, 99.431, 204.879 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.860 - 2.650 |
| R-factor | 0.19507 |
| Rwork | 0.193 |
| R-free | 0.22533 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3C8X 1shw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.225 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 18122 | |
| <I/σ(I)> | 35.74 | 5.509 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.8 | 21.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 290.9 | 14.9% PEG 4000, 0.1M Sodium citrate pH 5.6, 20% Isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 290.9K, pH 5.60 |
