3CYY
The crystal structure of ZO-1 PDZ2 in complex with the Cx43 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-04 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.040, 68.140, 149.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.21715 |
| Rwork | 0.215 |
| R-free | 0.25366 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | synaptojanin-2 binding protein (PDB id: 2JIN) and PICK1 (PDB id: 2GZV) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.216 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.160 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.051 |
| Number of reflections | 8265 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2M CaCl2, 0.1M HEPES, 14%(w/v) polyethylene glycol 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
