3CPW
The structure of the antibiotic LINEZOLID bound to the large ribosomal subunit of HALOARCULA MARISMORTUI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-10-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 211.730, 298.580, 575.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.700 |
| Rwork | 0.191 |
| R-free | 0.23000 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNX |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.054 | 0.412 |
| Number of reflections | 470299 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 95.6 | 84.2 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 292 | PEG6000, KCL, NH4CL, MGCl2, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MGCl2 | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 2 | MGCl2 | ||
| 4 | 1 | 2 | KCL | ||
| 5 | 1 | 2 | NH4CL | ||
| 6 | 1 | 2 | PEG6000 |
