3CMD

Crystal structure of peptide deformylase from VRE-E.faecium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-19
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 64 2 2
Unit cell lengths	149.470, 149.470, 143.329
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.2
Rwork	0.200
R-free	0.22900
Starting model (for MR)	1lm6
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (within the CCP4 program suite)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	25754
<I/σ(I)>	12.2	3.45
Completeness [%]	75.0	97.4
Redundancy	7.8	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	295	Na-malonate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K