3CDZ

Crystal structure of human factor VIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	134.113, 134.113, 349.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.980
R-factor	0.259
Rwork	0.256
R-free	0.32700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	homology models of individual domain built by SWISS-MODEL using PDB entries 1SDD 1KCW and 1D7P.
RMSD bond length	0.014
RMSD bond angle	1.735
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.980	3.980
Rmerge	0.130	0.827
Number of reflections	26444
<I/σ(I)>	27.2	1.7
Completeness [%]	99.0	92.3
Redundancy	13.2	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	100 mM Tris-HCl, 10% EtOH, 7% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K