3CBY
The Dvl2 PDZ Domain in Complex with the N1 Inhibitory Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.303, 52.187, 31.911 |
| Unit cell angles | 90.00, 100.33, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.136 |
| Rwork | 0.134 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cbx |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.513 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.065 | 0.042 | 0.636 |
| Number of reflections | 34942 | ||
| <I/σ(I)> | 19.3 | 2.6 | |
| Completeness [%] | 98.3 | 98.7 | 96.8 |
| Redundancy | 5.1 | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 1.6 M tri-sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
