3C7K
Molecular architecture of Galphao and the structural basis for RGS16-mediated deactivation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-01 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.15 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 96.357, 96.357, 235.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| Rwork | 0.250 |
| R-free | 0.31100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1agr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.346 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 27489 |
| <I/σ(I)> | 20.2 |
| Completeness [%] | 83.0 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 15% PEG 8000 200 mM Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
