3BT7
Structure of E. coli 5-Methyluridine Methyltransferase TrmA in complex with 19 nucleotide T-arm analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.407, 70.131, 107.950 |
| Unit cell angles | 90.00, 120.88, 90.00 |
Refinement procedure
| Resolution | 35.070 - 2.430 |
| R-factor | 0.2297 |
| Rwork | 0.225 |
| R-free | 0.28635 |
| Structure solution method | SAD |
| Starting model (for MR) | 2bh2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.781 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.100 | 2.490 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.078 | 0.270 |
| Number of reflections | 42023 | |
| <I/σ(I)> | 5.7 | 1.7 |
| Completeness [%] | 83.7 | |
| Redundancy | 2.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | 100 mM sodium cacodylate pH 7.4, 200 mM sodium sulfate, and 22 % PEG-8000, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium cacodylate | ||
| 2 | 1 | 2 | sodium cacodylate | ||
| 3 | 1 | 1 | sodium sulfate | ||
| 4 | 1 | 2 | sodium sulfate | ||
| 5 | 1 | 1 | PEG-8000 | ||
| 6 | 1 | 2 | PEG-8000 |
