3BT2

Structure of urokinase receptor, urokinase and vitronectin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	52.096, 87.205, 124.274
Unit cell angles	90.00, 94.31, 90.00

Refinement procedure

Resolution	42.880 - 2.500
R-factor	0.22993
Rwork	0.229
R-free	0.27170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fd6
RMSD bond length	0.018
RMSD bond angle	1.740
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.077	0.356
Number of reflections	38381
<I/σ(I)>	24.1	2.8
Completeness [%]	99.2	96
Redundancy	5.3	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.5	295	8% PEG 4000, 2.5% ethanol, 0.05% sodium azide, 50mM cacodylate pH 6.5, pH 7.5, MICRODIALYSIS, temperature 295K
1	MICRODIALYSIS	7.5	295	8% PEG 4000, 2.5% ethanol, 0.05% sodium azide, 50mM cacodylate pH 6.5, pH 7.5, MICRODIALYSIS, temperature 295K
1	MICRODIALYSIS	7.5	295	8% PEG 4000, 2.5% ethanol, 0.05% sodium azide, 50mM cacodylate pH 6.5, pH 7.5, MICRODIALYSIS, temperature 295K