3BHH
Crystal structure of human calcium/calmodulin-dependent protein kinase IIB isoform 1 (CAMK2B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97649 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.400, 79.200, 98.700 |
| Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
| Resolution | 47.780 - 2.400 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v7o |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.562 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.780 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.116 | 0.443 |
| Number of reflections | 50690 | |
| <I/σ(I)> | 9.77 | 2.69 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 3.76 | 3.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 50% PEG 300, 0.2M NaCl, 0.1M Na/KPO4 pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
