3BFT

Structure of the ligand-binding core of GluR2 in complex with the agonist (S)-TDPA at 2.25 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	110
Detector technology	CCD
Collection date	2003-08-19
Detector	MAR CCD 165 mm
Wavelength(s)	0.9322
Spacegroup name	P 21 21 2
Unit cell lengths	113.820, 163.333, 47.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.270
Rwork	0.200
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mdq
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.115	0.529
Number of reflections	41248
<I/σ(I)>	7.9	2.3
Completeness [%]	99.2	98.5
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	279	PEG 2000, cacodylate, zinc acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K