3BA0

Crystal structure of full-length human MMP-12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-25
Detector	OXFORD ONYX CCD
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	135.037, 60.148, 59.611
Unit cell angles	90.00, 90.74, 90.00

Refinement procedure

Resolution	30.700 - 3.000
R-factor	0.24339
Rwork	0.236
R-free	0.31900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y93 1su3
RMSD bond length	0.049
RMSD bond angle	4.271
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.700	3.160
High resolution limit [Å]	3.000	3.000
Rmerge	0.150	0.430
Number of reflections	9308
<I/σ(I)>	4.9	1.8
Completeness [%]	97.8	97.7
Redundancy	4.8	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	0.1M Tris-HCl, 30% PEG8000, 200mM acetohydroxamic acid, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K