3B7O
Crystal structure of the human tyrosine phosphatase SHP2 (PTPN11) with an accessible active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00721 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.813, 86.496, 166.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.120 - 1.600 |
| R-factor | 0.17204 |
| Rwork | 0.170 |
| R-free | 0.20897 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2shp |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.593 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.120 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.092 | 1.010 |
| Number of reflections | 43184 | |
| <I/σ(I)> | 13.3 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG 3350, 0.15 M Na Malate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
