3B6W
Crystal Structure of the GLUR2 Ligand Binding Core (S1S2J) T686S Mutant in Complex with Glutamate at 1.7 Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.817, 95.840, 122.886 |
| Unit cell angles | 90.00, 93.43, 90.00 |
Refinement procedure
| Resolution | 123.000 - 1.700 |
| R-factor | 0.22677 |
| Rwork | 0.225 |
| R-free | 0.26007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.438 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.000 | 1.744 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 119308 | |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-HCl, 0.15-0.2 M lithium sulfate, 18-21% PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
