3AWI
Bifunctional tRNA modification enzyme MnmC from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.138, 243.022, 175.527 |
| Unit cell angles | 90.00, 90.42, 90.00 |
Refinement procedure
| Resolution | 46.068 - 3.000 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.25140 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 151613 | |
| Completeness [%] | 98.3 | 94 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-Tris (pH 5.5), 25% PEG 3350, 0.25M ammonium sulfate, 10mM hexamine cobalt(III), VAPOR DIFFUSION, SITTING DROP, temperature 293K |
