3AL6
Crystal structure of Human TYW5
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.48505 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 165.871, 165.871, 105.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.017 - 2.800 |
| R-factor | 0.2427 |
| Rwork | 0.240 |
| R-free | 0.29540 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
| Rmerge | 0.082 | 0.050 | 0.480 |
| Number of reflections | 36950 | ||
| <I/σ(I)> | 56.444 | ||
| Completeness [%] | 99.6 | 95.8 | 98.1 |
| Redundancy | 19 | 22 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 200mM potassium thiocyanate, 10% (w/v) PEG3350, pH 8.0, vapor diffusion, sitting drop, temperature 293K |
