3A4K
Crystal structural analysis of HindIII restriction endonuclease in complex with cognate DNA and divalent cations at 2.17 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.460, 132.211, 94.070 |
| Unit cell angles | 90.00, 111.03, 90.00 |
Refinement procedure
| Resolution | 41.670 - 2.170 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e52 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.779 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.063 | 0.404 |
| Number of reflections | 100664 | |
| <I/σ(I)> | 24.7 | 3.5 |
| Completeness [%] | 99.8 | 98 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, ammonium acetate, Magnesium ion, glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
