3A4K
Crystal structural analysis of HindIII restriction endonuclease in complex with cognate DNA and divalent cations at 2.17 angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, C, B, D) | Type-2 restriction enzyme HindIII | polymer | 301 | 35136.3 | 4 | UniProt (P43870) Pfam (PF09518) | Haemophilus influenzae | HindIIIR, R.HindIII, Type II restriction enzyme HindIII, Endonuclease HindIII |
| 2 | E, G, I, K (E, G, I, K) | DNA (5'-D(*GP*CP*CP*A)-3') | polymer | 4 | 1175.8 | 4 | |||
| 3 | F, H, J, L (F, H, J, L) | DNA (5'-D(P*AP*GP*CP*TP*TP*GP*GP*C)-3') | polymer | 8 | 2442.6 | 4 | |||
| 4 | M, N (M, N) | DNA (5'-D(*GP*CP*CP*AP*AP*GP*CP*TP*TP*GP*GP*C)-3') | polymer | 12 | 3663.4 | 2 | |||
| 5 | O, R, U, Y (A, C, B, D) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 6 | P, S, V, Z (A, C, B, D) | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) | |||
| 7 | AA, Q (D, A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 8 | BA, T, W, X (D, C, B) | ACETATE ION | non-polymer | 59.0 | 4 | Chemie (ACT) | |||
| 9 | CA, DA, EA, FA, GA... (A, C, B, D, E...) | water | water | 18.0 | 573 | Chemie (HOH) |
Sequence modifications
A, C, B, D: 0 - 299 (UniProt: P43870)
| PDB | External Database | Details |
|---|---|---|
| His -1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 162345.8 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 737.3 | |
| All* | Total formula weight | 163083.1 |
