2E52

Crystal structural analysis of HindIII restriction endonuclease in complex with cognate DNA at 2.0 angstrom resolution

Summary for 2E52

• Entry DOI: 10.2210/pdb2e52/pdb
• Descriptor: Type II restriction enzyme HindIII, DNA (5'-D(*DGP*DCP*DCP*DAP*DAP*DGP*DCP*DTP*DTP*DGP*DGP*DC)-3'), GLYCEROL, ... (5 entities in total)
• Functional Keywords: type ii restriction enzyme hindiii(e.c.3.1.21.4)/dna, hydrolase-dna complex, hydrolase/dna
• Biological source: Haemophilus influenzae
• Total number of polymer chains: 10
• Total formula weight: 162518.55

Authors

Watanabe, N.,Sato, C.,Tanaka, I. (deposition date: 2006-12-18, release date: 2007-12-18, Last modification date: 2024-03-13)

Primary citation

Watanabe, N.,Takasaki, Y.,Sato, C.,Ando, S.,Tanaka, I.
Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations
Acta Crystallogr.,Sect.D, 65:1326-1333, 2009

PubMed Abstract: The three-dimensional crystal structures of HindIII bound to its cognate DNA with and without divalent cations were solved at 2.17 and 2.00 A resolution, respectively. HindIII forms a dimer. The structures showed that HindIII belongs to the EcoRI-like (alpha-class) subfamily of type II restriction endonucleases. The cognate DNA-complex structures revealed the specific DNA-recognition mechanism of HindIII by which it recognizes the palindromic sequence A/AGCTT. In the Mg(2+) ion-soaked structure the DNA was cleaved and two ions were bound at each active site, corresponding to the two-metal-ion mechanism.

PubMed: 19966419
DOI: 10.1107/S0907444909041134

Experimental method

X-RAY DIFFRACTION (2 Å)