37LU
NPC1-NPC2 complex with bis-sterol molecule JM046, pH 5.5
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| Number of Particle | 32074444 |
| Software | RELION,PHENIX,SerialEM,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
