2ZVM
Crystal structure of PCNA in complex with DNA polymerase iota fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 167.620, 68.820, 90.180 |
Unit cell angles | 90.00, 95.05, 90.00 |
Refinement procedure
Resolution | 19.950 - 2.300 |
R-factor | 0.19627 |
Rwork | 0.193 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vym |
RMSD bond length | 0.020 |
RMSD bond angle | 1.790 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.067 | 0.338 |
Number of reflections | 44815 | |
<I/σ(I)> | 13.5 | 3.2 |
Completeness [%] | 98.0 | 86.4 |
Redundancy | 4.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | HANGING DROP VAPOR DIFFUSION | 6.4 | 293 | pH6.4, HANGING DROP VAPOR DIFFUSION, temperature 293K |