2ZU6

crystal structure of the eIF4A-PDCD4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	113
Detector technology	CCD
Collection date	2008-04-20
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 2
Unit cell lengths	156.893, 165.384, 100.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.25003
Rwork	0.248
R-free	0.29363
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.446
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXS
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Rmerge	0.094
Number of reflections	64611
<I/σ(I)>	26.8
Completeness [%]	99.9
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	14% MPD, 0.1M Sodium Citrate, pH6.0, 100mM Ammonium acetate, 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277K