2ZO9
Malonate-bound structure of the glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) and characterization of the native Fe2+ metal ion preference
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2947 |
Spacegroup name | P 21 3 |
Unit cell lengths | 164.159, 164.159, 164.159 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.030 - 2.200 |
R-factor | 0.17059 |
Rwork | 0.170 |
R-free | 0.18906 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dxn |
RMSD bond length | 0.014 |
RMSD bond angle | 1.602 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.340 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 73371 | |
<I/σ(I)> | 20.9 | 1.6 |
Completeness [%] | 98.2 | 94.1 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 277 | pH 7, VAPOR DIFFUSION, temperature 277K |