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2ZKF

Crystal structure of the SRA domain of mouse Np95 in complex with hemi-methylated CpG DNA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPHOTON FACTORY BEAMLINE BL-5A
Synchrotron sitePhoton Factory
BeamlineBL-5A
Temperature [K]100
Detector technologyCCD
Collection date2008-01-25
DetectorADSC QUANTUM 315
Wavelength(s)1.0
Spacegroup nameP 41 21 2
Unit cell lengths58.878, 58.878, 182.309
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution49.460 - 2.550
R-factor0.21716
Rwork0.214
R-free0.27450
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2zkd
RMSD bond length0.017
RMSD bond angle2.012
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.640
High resolution limit [Å]2.5502.550
Rmerge0.0790.171
Number of reflections11160
Completeness [%]99.6100
Redundancy9.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.52930.1M Bis-Tris-propane (pH7.5), 0.2M sodium fluoride, 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111Bis-Tris-propane
211sodium fluoride
311PEG3350
411HOH
512Bis-Tris-propane
612sodium fluoride
712PEG3350
812HOH

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PDB entries from 2024-10-16

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