2ZKF
Crystal structure of the SRA domain of mouse Np95 in complex with hemi-methylated CpG DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 58.878, 58.878, 182.309 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.460 - 2.550 |
R-factor | 0.21716 |
Rwork | 0.214 |
R-free | 0.27450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zkd |
RMSD bond length | 0.017 |
RMSD bond angle | 2.012 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.079 | 0.171 |
Number of reflections | 11160 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Bis-Tris-propane (pH7.5), 0.2M sodium fluoride, 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Bis-Tris-propane | ||
2 | 1 | 1 | sodium fluoride | ||
3 | 1 | 1 | PEG3350 | ||
4 | 1 | 1 | HOH | ||
5 | 1 | 2 | Bis-Tris-propane | ||
6 | 1 | 2 | sodium fluoride | ||
7 | 1 | 2 | PEG3350 | ||
8 | 1 | 2 | HOH |