2ZKF
Crystal structure of the SRA domain of mouse Np95 in complex with hemi-methylated CpG DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.878, 58.878, 182.309 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.460 - 2.550 |
| R-factor | 0.21716 |
| Rwork | 0.214 |
| R-free | 0.27450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zkd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.012 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.079 | 0.171 |
| Number of reflections | 11160 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Bis-Tris-propane (pH7.5), 0.2M sodium fluoride, 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Bis-Tris-propane | ||
| 2 | 1 | 1 | sodium fluoride | ||
| 3 | 1 | 1 | PEG3350 | ||
| 4 | 1 | 1 | HOH | ||
| 5 | 1 | 2 | Bis-Tris-propane | ||
| 6 | 1 | 2 | sodium fluoride | ||
| 7 | 1 | 2 | PEG3350 | ||
| 8 | 1 | 2 | HOH |
