2Z8C

Phosphorylated insulin receptor tyrosine kinase in complex with (4-{[5-carbamoyl-4-(3-methylanilino)pyrimidin-2-yl]amino}phenyl)acetic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Detector technology	DIFFRACTOMETER
Collection date	2001-01-01
Detector	WEISSENBERG
Spacegroup name	P 32 2 1
Unit cell lengths	71.540, 71.540, 133.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.230 - 3.250
Rwork	0.217
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ir3
RMSD bond length	0.010
RMSD bond angle	1.800
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNX
Refinement software	CNX (2002)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.230
High resolution limit [Å]	3.250
Rmerge	0.092
Number of reflections	6395
Completeness [%]	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			PEG 3350, VAPOR DIFFUSION, HANGING DROP