2Z3Z
Prolyl tripeptidyl aminopeptidase mutant E636A complexd with an inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 149.825, 149.825, 161.506 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.18419 |
Rwork | 0.183 |
R-free | 0.21188 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2z3w |
RMSD bond length | 0.015 |
RMSD bond angle | 1.386 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.062 | 0.304 |
Number of reflections | 77814 | |
<I/σ(I)> | 83.8 | 11 |
Completeness [%] | 99.8 | 99 |
Redundancy | 20 | 18.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 1.1M pottasium sodium tartrate, 0.2M lithium sulfate, 0.1M CHES buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |