2YPQ
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase with tryptophan and tyrosine bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RAXIS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 202.777, 202.777, 66.747 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 175.670 - 2.760 |
| R-factor | 0.18081 |
| Rwork | 0.177 |
| R-free | 0.24888 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nv8 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.348 |
| Data reduction software | d*TREK |
| Data scaling software | CrystalClear (1.3.6) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.720 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.130 | 0.450 |
| Number of reflections | 39770 | |
| <I/σ(I)> | 7.2 | 2.4 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 4.06 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS-HCL PH 7.5, 1.5 M AMMONIUM SULFATE, 15% V/V GLYCEROL |
