2YPP
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase in complex with 3 tyrosine molecules
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 203.449, 203.449, 66.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.020 - 2.300 |
| R-factor | 0.14046 |
| Rwork | 0.139 |
| R-free | 0.16375 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nv8 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.058 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.030 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.130 | 0.240 |
| Number of reflections | 67961 | |
| <I/σ(I)> | 10.7 | 3.1 |
| Completeness [%] | 97.0 | 86.7 |
| Redundancy | 7.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M TRIS-HCL PH 7.5, 1.5 M AMMONIUM SULFATE, 12% V/V GLYCEROL |
