2YLF
Crystal structure of the human Spir-1 KIND domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.914, 65.914, 78.803 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.960 - 2.050 |
| R-factor | 0.20283 |
| Rwork | 0.200 |
| R-free | 0.26339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yle |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.807 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.170 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.080 | 0.420 |
| Number of reflections | 11995 | |
| <I/σ(I)> | 14.1 | 2.8 |
| Completeness [%] | 97.5 | 94.2 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
