2YKG

Structural insights into RNA recognition by RIG-I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	173
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	47.618, 76.208, 219.825
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.500
R-factor	0.223
Rwork	0.220
R-free	0.27310
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.339
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	2.500
Rmerge	0.080
Number of reflections	26861
<I/σ(I)>	14.5
Completeness [%]	93.0
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9		0.1 M BICINE, PH 9.0, 22.5 % POLYETHYLENE GLYCOL 6,000