2YHY
Structure of N-Acetylmannosamine kinase in complex with N- acetylmannosamine and ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 90.380, 90.380, 101.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.260 - 1.820 |
R-factor | 0.15372 |
Rwork | 0.152 |
R-free | 0.18163 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eo3 |
RMSD bond length | 0.025 |
RMSD bond angle | 2.097 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.050 | 0.690 |
Number of reflections | 489925 | |
<I/σ(I)> | 34.07 | 4.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M CALCIUM ACETATE, 0.1M SODIUM CACODYLATE PH 6.5, 40% PEG 300. |