2YHY
Structure of N-Acetylmannosamine kinase in complex with N- acetylmannosamine and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.380, 90.380, 101.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.260 - 1.820 |
| R-factor | 0.15372 |
| Rwork | 0.152 |
| R-free | 0.18163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eo3 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.097 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.050 | 0.690 |
| Number of reflections | 489925 | |
| <I/σ(I)> | 34.07 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M CALCIUM ACETATE, 0.1M SODIUM CACODYLATE PH 6.5, 40% PEG 300. |
