2YHO
The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.690, 137.870, 63.750 |
| Unit cell angles | 90.00, 106.39, 90.00 |
Refinement procedure
| Resolution | 55.906 - 2.100 |
| R-factor | 0.1881 |
| Rwork | 0.186 |
| R-free | 0.23330 |
| Structure solution method | MIR |
| Starting model (for MR) | PDB ENTRIES 3EB6 2yhn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.153 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.910 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 53163 | |
| <I/σ(I)> | 9 | 3.2 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M SODIUM CITRATE PH 5.5, 0.2 M SODIUM ACETATE, 10% PEG 4000 |
