2YEO
A39L mutation of scorpion toxin lqh-alpha-it
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-01 |
| Detector | ADSC CCD |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 129.707, 129.707, 129.707 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.003 - 1.080 |
| R-factor | 0.1205 |
| Rwork | 0.120 |
| R-free | 0.12340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2asc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.694 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.100 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.060 | 0.770 |
| Number of reflections | 694937 | |
| <I/σ(I)> | 48.6 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 17.2 | 17.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.01 M HEXADECYLTRIMETHYLAMMONIUM BROMIDE, 0.5 M SODIUM CHLORIDE, 0.01 M MAGNESIUM CHLORIDE HEXAHYDRATE PROTEIN MOLECULE WAS OF 10MG/ML DISSOLVED IN DISTILLED WATER |
