2YDX

Crystal structure of human S-adenosylmethionine synthetase 2, beta subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-19
Detector	ADSC CCD
Spacegroup name	P 42 21 2
Unit cell lengths	163.389, 163.389, 252.882
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.960 - 2.800
R-factor	0.1686
Rwork	0.168
R-free	0.18820
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.960	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.050	1.030
Number of reflections	78936
<I/σ(I)>	9.8	1.1
Completeness [%]	93.2	56
Redundancy	6.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1