2YDW
Crystal Structure of the First Bromodomain of Human Brd2 with the inhibitor GW841819X
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 100 |
| Collection date | 1996-06-01 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.372, 56.013, 67.425 |
| Unit cell angles | 90.00, 93.98, 90.00 |
Refinement procedure
| Resolution | 67.270 - 1.900 |
| R-factor | 0.20247 |
| Rwork | 0.200 |
| R-free | 0.24959 |
| Structure solution method | MIR |
| Starting model (for MR) | 1e6i |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.101 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.260 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 34166 | |
| <I/σ(I)> | 11.5 | 3.3 |
| Completeness [%] | 98.8 | 94.6 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 17.5% PEG 3350, 0.2 M (NH4)2SO4, 0.1 M BIS TRIS PH 5.5. |
