2YDR
CpOGA D298N in complex with p53-derived O-GlcNAc peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 117.950, 117.950, 148.121 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.750 |
| R-factor | 0.18579 |
| Rwork | 0.185 |
| R-free | 0.21881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j62 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.425 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.150 | 0.580 |
| Number of reflections | 28111 | |
| <I/σ(I)> | 7.5 | 4.3 |
| Completeness [%] | 92.5 | 94.2 |
| Redundancy | 4.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.6 M NAAC, 0.175 M CDSO4, 0.1 M HEPES PH 7.5 |
