2Y7H
Atomic model of the DNA-bound methylase complex from the Type I restriction-modification enzyme EcoKI (M2S1). Based on fitting into EM map 1534.
EM specimen
Staining | Yes |
Staining material | Uranyl Acetate |
EM Data Collection
Microscope | JEOL 1200EX |
Electron Gun | TUNGSTEN HAIRPIN |
Accelerating Voltage (kV) | 80 |
Cs (mm) | 2 |
Detector | KODAK SO-163 FILM |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 18 |
Software | Situs,UCSF Chimera,URO,EMAN,IMAGIC,MRC IMAGE PROCESSING PACKAGE |
Symmetry | C2 |