2Y1N
Structure of c-Cbl-ZAP-70 peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-08 |
Detector | ADSC CCD |
Spacegroup name | P 65 |
Unit cell lengths | 93.570, 93.570, 189.599 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.423 - 1.999 |
R-factor | 0.159 |
Rwork | 0.158 |
R-free | 0.18040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cbl |
RMSD bond length | 0.008 |
RMSD bond angle | 1.142 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.520 |
Number of reflections | 63238 | |
<I/σ(I)> | 30.3 | 2.5 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 18-20% (W/V) PEG 3350, 0.2 M AMMONIUM FORMATE |