2Y04
TURKEY BETA1 ADRENERGIC RECEPTOR WITH STABILISING MUTATIONS AND BOUND PARTIAL AGONIST SALBUTAMOL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.900, 62.000, 101.500 |
| Unit cell angles | 90.00, 109.90, 90.00 |
Refinement procedure
| Resolution | 95.400 - 3.050 |
| R-factor | 0.22484 |
| Rwork | 0.223 |
| R-free | 0.25518 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vt4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.438 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.600 | 3.210 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.120 | 0.510 |
| Number of reflections | 19354 | |
| <I/σ(I)> | 7.7 | 1.8 |
| Completeness [%] | 95.5 | 89.5 |
| Redundancy | 2.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | SITTING DROP VAPOUR DIFFUSION, PROTEIN (16.5MG/ML) IN 100MM NACL, 0.1MM EDTA, 1.0MM (R, S)-SALBUTAMOL, 1.0MG/ML CHS, 0.5% HEGA-10, 0.1M BICINE PH9.0, 28% PEG400 AT 4 DEGREES C |
