2XW9
Crystal Structure of Complement Factor D mutant S183A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.510, 49.550, 55.480 |
| Unit cell angles | 90.00, 105.64, 90.00 |
Refinement procedure
| Resolution | 53.430 - 1.200 |
| R-factor | 0.14528 |
| Rwork | 0.144 |
| R-free | 0.17605 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dsu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.456 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.900 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.070 | 0.250 |
| Number of reflections | 64058 | |
| <I/σ(I)> | 8 | 3.5 |
| Completeness [%] | 99.3 | 98.8 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6.0 |
