2XT3

HUMAN KIF7, A KINESIN INVOLVED IN HEDGEHOG SIGNALLING

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	93
Detector technology	CCD
Collection date	2010-09-14
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	45.920, 79.790, 94.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.704 - 1.882
R-factor	0.1816
Rwork	0.179
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b6v
RMSD bond length	0.010
RMSD bond angle	1.458
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.980
High resolution limit [Å]	1.880	1.880
Rmerge	0.070	0.260
Number of reflections	28661
<I/σ(I)>	16.1	6.1
Completeness [%]	99.2	100
Redundancy	5.7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		pH 6.0