2XQW
Structure of Factor H domains 19-20 in complex with complement C3d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-29 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 61 |
| Unit cell lengths | 103.554, 103.554, 141.211 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.753 - 2.306 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3d |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.750 |
| Number of reflections | 37791 | |
| <I/σ(I)> | 15.3 | 2.36 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 295 | 12-18% PEG 4000, 0.1 M HEPES, PH 7.5, AT 22 DEGREES C |
