2XNK
Structure and function of the Rad9-binding region of the DNA damage checkpoint adaptor TopBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.490, 290.280, 60.580 |
| Unit cell angles | 90.00, 89.85, 90.00 |
Refinement procedure
| Resolution | 51.347 - 2.600 |
| R-factor | 0.2079 |
| Rwork | 0.205 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xnh |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.806 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.910 | 2.740 |
| High resolution limit [Å] | 2.470 | 2.600 |
| Rmerge | 0.100 | 0.380 |
| Number of reflections | 40485 | |
| <I/σ(I)> | 4.96 | 1.99 |
| Completeness [%] | 97.2 | 98.8 |
| Redundancy | 2.63 | 2.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M TRIS-HCL PH 7.5, 0.4 M MGCL2, 25% PEG 4000, 4% GLYCEROL, 0.01 M SPERMIDINE TETRA-HCL |
