2XMN
High resolution snapshots of defined TolC open states present an iris- like movement of periplasmic entrance helices
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-12 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 134.124, 135.524, 136.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.941 - 2.850 |
| R-factor | 0.2733 |
| Rwork | 0.271 |
| R-free | 0.30930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ek9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.244 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.100 | 0.450 |
| Number of reflections | 56719 | |
| <I/σ(I)> | 5.1 | 1.7 |
| Completeness [%] | 89.7 | 87.7 |
| Redundancy | 5.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | PH 7.4 |
