2XMN

High resolution snapshots of defined TolC open states present an iris- like movement of periplasmic entrance helices

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-04-12
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	134.124, 135.524, 136.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.941 - 2.850
R-factor	0.2733
Rwork	0.271
R-free	0.30930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ek9
RMSD bond length	0.010
RMSD bond angle	1.244
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.900
High resolution limit [Å]	2.750	2.750
Rmerge	0.100	0.450
Number of reflections	56719
<I/σ(I)>	5.1	1.7
Completeness [%]	89.7	87.7
Redundancy	5.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		PH 7.4