2XI3
HCV-H77 NS5B Polymerase Complexed With GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2003-06-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.010, 91.898, 61.054 |
| Unit cell angles | 89.62, 99.74, 92.95 |
Refinement procedure
| Resolution | 34.654 - 1.700 |
| R-factor | 0.1712 |
| Rwork | 0.170 |
| R-free | 0.19760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1quv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.034 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.350 |
| Number of reflections | 121329 | |
| <I/σ(I)> | 14.5 | 2 |
| Completeness [%] | 95.5 | 94 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | MES 50MM PH 6.5, AMMONIUM SULFATE 0.2M, AMMONIUM ACETATE 0.25M, PEG 1000 25%-30% |
